Blaine Pfeifer

PhD

Blaine Pfeifer

PhD

Blaine Pfeifer

PhD

Research Topics

Metabolic Engineering; heterologous natural product biosynthesis; genetic vaccine design

Contact Information

904 Furnas Hall

Buffalo NY, 14260

Phone: (716) 645-1198

Fax: (716) 645-3822

blainepf@buffalo.edu

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Publications

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  • U. Chitgupi, Y. Li, M. F. Chen, S. Shao, M. Beitelshees, M. J. Tan, S. Neelamegham, B. A. Pfeifer, C. Jones, J. F. Lovell, “Bimodal Targeting Using Sulfonated, Mannosylated PEI for Combined Gene Delivery and Photodynamic Therapy”, Photochem. Photobiol. 93, 600-608 (2017). link
  • E. K. Matich, D. M. Butryn, M. Ghafari, V. del solar, E. Camgoz, B. A. Pfeifer, D. S. Aga, B. Z. Haznedaroglu, G. E. Atilla-gokcumen, “Mass spectrometry-based metabolomics of value-added biochemicals from Ettlia oleoabundans”, Algal Res. 19, 146-154 (2016). link
  • M. K. Ahmadi, M. Ghafari, J. D. Atkinson, B. A. Pfeifer, “A copper removal process for water based upon biosynthesis of yersiniabactin, a metal-binding natural product”, Chem. Eng. J. 306, 772-776 (2016). link
  • M. K. Ahmadi, S. Fawaz, B. A. Pfeifer, “An aqueous two-phase system to pre-purify a heterologously produced siderophore”, Technology 4, 135-138 (2016). link
  • Y. Li, M. Beitelshees, L. Fang, A. Hill, M. K. Ahmadi, M. Chen, B. A. Davidson, P. Knight, R. J. Smith, S. T. Andreadis, A. P. Hakansson, C. H. Jones, B. A. Pfeifer, “In situ pneumococcal vaccine production and delivery through a hybrid biological-biomaterial vector”, Sci. Adv. 2, e1600264 (2016). link
  • M. K. Ahmadi, S. Fawaz, L. Fang, Z. P. Yu, B. A. Pfeifer, “Molecular variation of the nonribosomal peptide-polyketide siderophore yersiniabactin through biosynthetic and metabolic engineering”, Biotechnol. Bioeng. 113, 1067-1074 (2016). link
  • H. R. Zhang, L. Fang, M. S. Osborne, B. A. Pfeifer, “The Continuing Development of E. coli as a Heterologous Host for Complex Natural Product Biosynthesis”, Methods Mol. Biol. 1401, 121-134 (2016). link
  • A. B. Hi, M. F. Chen, C. K. Chen, B. A. Pfeifer, C. H. Jones, “Overcoming Gene-Delivery Hurdles: Physiological Considerations for Nonviral Vectors”, Trends Biotechnol. 34, 91-105 (2016). link
  • J. Kumpfmuller, K. Methling, L. Fang, B. A. Pfeifer, M. Lalk, T. Schweder, “Production of the polyketide 6-deoxyerythronolide B in the heterologous host Bacillus subtilis”, Appl. Microbiol. Biotechnol. 100, 1209-1220 (2016). link
  • Y. Li, G. J. Zhang, B. A. Pfeifer, “Current and Emerging Options for Taxol Production”, Adv. Biochem. Eng. Biotechnol. 148, 405-425 (2015). link
  • C. H. Jones, A. Hill, M. F. Chen, B. A. Pfeifer, “Contemporary Approaches for Nonviral Gene Therapy”, Discov. Med. 107, 447-454 (2015).
  • M. K. Ahmadi, S. Fawaz, C. H. Jones, G. J. Zhang, B. A. Pfeifer, “Total Biosynthesis and Diverse Applications of the Nonribosomal Peptide-Polyketide Siderophore Yersiniabactin”, Appl. Environ. Microbiol. 81, 5290-5298 (2015). link
  • C. H. Jones, A. Gollakota, M. F. Chen, T. C. Chung, A. Ravikrishnan, G. J. Zhang, B. A. Pfeifer, “Influence of molecular weight upon mannosylated bio-synthetic hybrids for targeted antigen presenting cell gene delivery”, Biomaterials 58, 103-111 (2015). link
  • T. C. Chung, C. H. Jones, A. Gollakota, M. K. Ahmadi, S. Rane, G. J. Zhang, B. A. Pfeifer, “Improved Escherichia coli Bactofection and Cytotoxicity by Heterologous Expression of Bacteriophage Phi X174 Lysis Gene E”, Mol. Pharm. 12, 1691-1700 (2015). link
  • C. H. Jones, M. F. Chen, A. Gollakota, A. Ravikrishnan, G. J. Zhang, S. Lin, M. Tan, C. Cheng, H. Q. Lin, B. A. Pfeifer, “Structure Function Assessment of Mannosylated Poly(beta-amino esters) upon Targeted Antigen Presenting Cell Gene Delivery”, Biomacromolecules 16, 1534-1541 (2015). link
  • C. H. Jones, C. K. Chen, M. F. Chen, A. Ravikrishnan, H. G. Zhang, A. Gollakota, T. C. Chung, C. Cheng, B. A. Pfeifer, “PEGylated Cationic Polylactides for Hybrid Biosynthetic Gene Delivery”, Mol. Pharm. 12, 846-856 (2015). link
  • C. H. Jones, M. F. Chen, A. Ravikrishnan, R. Reddinger, G. J. Zhang, A. P. Hakansson, B. A. Pfeifer, “Mannosylated poly(beta-amino esters) for targeted antigen presenting cell immune modulation”, Biomaterials 37, 333-344 (2015). link
  • C. H. Jones, A. P. Hakansson, B. A. Pfeifer, “Biomaterials at the interface of nano- and micro-scale vector-cellular interactions in genetic vaccine design”, J. Mat. Chem. B 2, 8053-8068 (2014). link
  • C. H. Jones, A. Ravikrishnan, M. F. Chen, R. Reddinger, M. K. Ahmadi, S. Rane, A. P. Hakansson, B. A. Pfeifer, “Hybrid biosynthetic gene therapy vector development and dual engineering capacity”, Proc. Natl. Acad. Sci. U. S. A. 111, 12360-12365 (2014). link
  • Y. Li, B. A. Pfeifer, “Heterologous production of plant-derived isoprenoid products in microbes and the application of metabolic engineering and synthetic biology”, Curr. Opin. Plant Biol. 19, 8-13 (2014). link
  • K. A. Carter, S. Shao, M. I. Hoopes, D. Luo, B. Ahsan, V. M. Grigoryants, W. T. Song, H. Y. Huang, G. J. Zhang, R. K. Pandey, J. Geng, B. A. Pfeifer, C. P. Scholes, J. Ortega, M. Karttunen, J. F. Lovell, “Porphyrin-phospholipid liposomes permeabilized by near-infrared light”, Nat. Commun. 5, 3546 (2014). link
  • C. K. Chen, Q. Wang, C. H. Jones, Y. Yu, H. G. Zhang, W. C. Law, C. K. Lai, Q. H. Zeng, P. N. Prasad, B. A. Pfeifer, C. Cheng, “Synthesis of pH-Responsive Chitosan Nanocapsules for the Controlled Delivery of Doxorubicin”, Langmuir 30, 4111-4119 (2014). link
  • M. Jiang, H. R. Zhang, B. A. Pfeifer, “The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli”, J. Vis. Exp. , e4346 (2013). link
  • M. Jiang, H. R. Zhang, S. H. Park, Y. Li, B. A. Pfeifer, “Deoxysugar pathway interchange for erythromycin analogues heterologously produced through Escherichia coli”, Metab. Eng. 20, 92-100 (2013). link
  • C. K. Chen, C. H. Jones, P. Mistriotis, Y. Yu, X. N. Ma, A. Ravikrishnan, M. Jiang, S. T. Andreadis, B. A. Pfeifer, C. Cheng, “Poly(ethylene glycol)-block-cationic polylactide nanocomplexes of differing charge density for gene delivery”, Biomaterials 34, 9688-9699 (2013). link
  • C. H. Jones, S. Rane, E. Patt, A. Ravikrishnan, C. K. Chen, C. Cheng, B. A. Pfeifer, “Polymyxin B Treatment Improves Bactofection Efficacy and Reduces Cytotoxicity”, Mol. Pharm. 10, 4301-4308 (2013). link
  • C. H. Jones, C. K. Chen, A. Ravikrishnan, S. Rane, B. A. Pfeifer, “Overcoming Nonviral Gene Delivery Barriers: Perspective and Future”, Mol. Pharm. 10, 4082-4098 (2013). link
  • M. Jiang, B. A. Pfeifer, “Metabolic and pathway engineering to influence native and altered erythromycin production through E. coli”, Metab. Eng. 19, 42-49 (2013). link
  • M. Jiang, L. Fang, B. A. Pfeifer, “Improved heterologous erythromycin A production through expression plasmid re-design”, Biotechnol. Prog. 29, 862-869 (2013). link
  • C. H. Jones, C. K. Chen, M. Jiang, L. Fang, C. Cheng, B. A. Pfeifer, “Synthesis of Cationic Polylactides with Tunable Charge Densities as Nanocarriers for Effective Gene Delivery”, Mol. Pharm. 10, 1138-1145 (2013). link
  • J. Rucker, J. Paul, B. A. Pfeifer, K. Lee, “Engineering E. coli for triglyceride accumulation through native and heterologous metabolic reactions”, Appl. Microbiol. Biotechnol. 97, 2753-2759 (2013). link
  • M. Jiang, G. Stephanopoulos, B. A. Pfeifer, “Downstream reactions and engineering in the microbially reconstituted pathway for Taxol”, Appl. Microbiol. Biotechnol. 94, 841-849 (2012). link
  • M. Jiang, G. Stephanopoulos, B. A. Pfeifer, “Toward Biosynthetic Design and Implementation of Escherichia coli-Derived Paclitaxel and Other Heterologous Polyisoprene Compounds”, Appl. Environ. Microbiol. 78, 2497-2504 (2012). link
  • J. W. Armando, B. A. Boghigian, B. A. Pfeifer, “LC-MS/MS quantification of short-chain acyl-CoA's in Escherichia coli demonstrates versatile propionyl-CoA synthetase substrate specificity”, Lett. Appl. Microbiol. 54, 140-148 (2012). link
  • H. Zhang, B. A. Boghigian, J. Armando, B. A. Pfeifer, “Methods and options for the heterologous production of complex natural products”, Nat. Prod. Rep. 28, 125-151 (2011). link
  • B. A. Boghigian, H. Zhang, B. A. Pfeifer, “Multi-Factorial Engineering of Heterologous Polyketide Production in Escherichia coli Reveals Complex Pathway Interactions”, Biotechnol. Bioeng. 108, 1360-1371 (2011). link
  • B. A. Boghigian, K. Lee, B. A. Pfeifer, “Computational analysis of phenotypic space in heterologous polyketide biosynthesis-Applications to Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae”, J. Theor. Biol. 262, 197-207 (2010). link
  • H. Zhang, B. A. Boghigian, B. A. Pfeifer, “Investigating the Role of Native Propionyl-CoA and Methylmalonyl-CoA Metabolism on Heterologous Polyketide Production in Escherichia coli”, Biotechnol. Bioeng. 105, 567-573 (2010). link
  • B. A. Boghigian, G. Seth, R. Kiss, B. A. Pfeifer, “Metabolic flux analysis and pharmaceutical production”, Metab. Eng. 12, 81-95 (2010). link
  • B. A. Boghigian, H. Shi, K. Lee, B. A. Pfeifer, “Utilizing elementary mode analysis, pathway thermodynamics, and a genetic algorithm for metabolic flux determination and optimal metabolic network design”, BMC Syst. Biol. 4, 49 (2010). link
  • J. Wu, B. A. Boghigian, M. Myint, H. Zhang, S. Zhang, B. A. Pfeifer, “Construction and performance of heterologous polyketide-producing K-12-and B-derived Escherichia coli”, Lett. Appl. Microbiol. 51, 196-204 (2010). link
  • P. K. Ajikumar, W. H. Xiao, K. E. J. Tyo, Y. Wang, F. Simeon, E. Leonard, O. Mucha, T. H. Phon, B. Pfeifer, G. Stephanopoulos, “Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli”, Science 330, 70-74 (2010). link
  • H. Zhang, Y. Wang, J. Q. Wu, K. Skalina, B. A. Pfeifer, “Complete Biosynthesis of Erythromycin A and Designed Analogs Using E. coli as a Heterologous Host”, Chem. Biol. 17, 1232-1240 (2010). link
  • H. Zhang, Y. Wang, B. Boghigian, B. A. Pfeifer, “Probing the heterologous metabolism supporting 6-deoxyerythronolide B biosynthesis in Escherichia coli”, Microb. Biotechnol. 2, 390-394 (2009). link
  • M. Pistorino, B. A. Pfeifer, “Efficient Experimental Design and Micro-Scale Medium Enhancement of 6-deoxyerythronolide B Production through Escherichia coli”, Biotechnol. Prog. 25, 1364-1371 (2009). link
  • Y. Wang, B. A. Pfeifer, “6-Deoxyerythronolide B production through chromosomal localization of the deoxyerythronolide B synthase genes in E. coli”, Metab. Eng. 10, 33-38 (2008). link
  • M. Pistorino, B. A. Pfeifer, “Polyketide analysis using mass spectrometry, evaporative light scattering, and charged aerosol detector systems”, Anal. Bioanal. Chem. 390, 1189-1193 (2008). link
  • H. Zhang, Y. Wang, B. A. Pfeifer, “Bacterial hosts for natural product production”, Mol. Pharm. 5, 212-225 (2008). link
  • B. Pfeifer, “Natural products and production systems: Opening comments”, Mol. Pharm. 5, 165-166 (2008). link
  • S. Parsa, Y. Wang, J. Fuller, R. Langer, B. A. Pfeifer, “A comparison between polymeric microsphere and bacterial vectors for macrophage P388D1 gene delivery”, Pharm. Res. 25, 1202-1208 (2008). link
  • B. A. Boghigian, B. A. Pfeifer, “Current status, strategies, and potential for the metabolic engineering of heterologous polyketides in Escherichia coli”, Biotechnol. Lett. 30, 1323-1330 (2008). link
  • S. Parsa, Y. Wang, K. Rines, B. A. Pfeifer, “A high-throughput comparison of recombinant gene expression parameters for E-coli-mediated gene transfer to P388D1 macrophage cells”, J. Biotechnol. 137, 59-64 (2008). link
  • S. Parsa, B. Pfeifer, “Engineering bacterial vectors for delivery of genes and proteins to antigen-presenting cells”, Mol. Pharm. 4, 4-17 (2007). link
  • Y. Wang, B. A. Boghigian, B. A. Pfeifer, “Improving heterologous polyketide production in Escherichia coli by overexpression of an S-adenosylmethionine synthetase gene”, Appl. Microbiol. Biotechnol. 77, 367-373 (2007). link
  • B. A. Pfeifer, J. A. Burdick, S. R. Little, R. Langer, “Poly(ester-anhydride): poly(beta-amino ester) micro- and nanospheres: DNA encapsulation and cellular transfection”, Int. J. Pharm. 304, 210-219 (2005). link

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