Sriram Neelamegham

PhD

Sriram Neelamegham

PhD

Sriram Neelamegham

PhD

Research Topics

Biomedical engineering; cell biomechanics; vascular engineering

Contact Information

906 Furnas Hall

Buffalo NY, 14260

Phone: (716) 645-1200

Fax: (716) 645-3822

neel@buffalo.edu

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Publications

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  • C. J. Zhang, S. Neelamegham, “Application of microfluidic devices in studies of thrombosis and hemostasis”, Platelets 28, 434-440 (2017). link
  • U. Chitgupi, Y. Li, M. F. Chen, S. Shao, M. Beitelshees, M. J. Tan, S. Neelamegham, B. A. Pfeifer, C. Jones, J. F. Lovell, “Bimodal Targeting Using Sulfonated, Mannosylated PEI for Combined Gene Delivery and Photodynamic Therapy”, Photochem. Photobiol. 93, 600-608 (2017). link
  • K. Cheng, Y. S. Zhou, S. Neelamegham, “DrawGlycan-SNFG: a robust tool to render glycans and glycopeptides with fragmentation information”, Glycobiology 27, 200-205 (2017). link
  • A. Buffone, M. Nasirikenari, C. T. Manhardt, A. Lugade, P. N. Bogner, R. Sackstein, Y. Thanavala, S. Neelamegham, J. T. Y. Lau, “Leukocyte-borne alpha(1,3)-fucose is a negative regulator of beta(2)-integrin-dependent recruitment in lung inflammation”, J. Leukoc. Biol. 101, 459-470 (2017). link
  • S. Neelamegham, L. K. Mahal, “Multi-level regulation of cellular glycosylation: from genes to transcript to enzyme to structure”, Curr. Opin. Struct. Biol. 40, 145-152 (2016). link
  • E. V. Chandrasekaran, J. Xue, J. Xia, S. D. Khaja, C. F. Piskorz, R. D. Locke, S. Neelamegham, K. L. Matta, “Novel interactions of complex carbohydrates with peanut (PNA), Ricinus communis (RCA-I), Sambucus nigra (SNA-I) and wheat germ (WGA) agglutinins as revealed by the binding specificities of these lectins towards mucin core-2 O-linked and N-linked glycans and related structures”, Glycoconjugate J. 33, 819-836 (2016). link
  • G. Stolfa, N. Mondal, Y. Q. Zhu, X. H. Yu, A. Buffone, S. Neelamegham, “Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion”, Sci Rep 6, 30392 (2016). link
  • A. Nascimbene, S. Neelamegham, O. H. Frazier, J. L. Moake, J. F. Dong, “Acquired von Willebrand syndrome associated with left ventricular assist device”, Blood 127, 3133-3141 (2016). link
  • N. Mondal, G. Stolfa, A. Antonopoulos, Y. Q. Zhu, S. S. Wang, A. Buffone, G. E. Atilla-gokcumen, S. M. Haslam, A. Dell, S. Neelamegham, “Glycosphingolipids on Human Myeloid Cells Stabilize E-Selectin-Dependent Rolling in the Multistep Leukocyte Adhesion Cascade”, Arterioscler. Thromb. Vasc. Biol. 36, 718-727 (2016). link
  • S. Gogia, S. Neelamegham, “Role of fluid shear stress in regulating VWF structure, function and related blood disorders”, Biorheology 52, 319-335 (2016). link
  • R. Gupta, K. L. Matta, S. Neelamegham, “A systematic analysis of acceptor specificity and reaction kinetics of five human alpha(2,3)sialyltransferases: Product inhibition studies illustrate reaction mechanism for ST3Gal-I”, Biochem. Biophys. Res. Commun. 469, 606-612 (2016). link
  • C. Y. Lo, B. R. Weil, B. A. Palka, A. Momeni, J. M. Canty, S. Neelamegham, “Cell surface glycoengineering improves selectin-mediated adhesion of mesenchymal stem cells (MSCs) and cardiosphere-derived cells (CDCs): Pilot validation in porcine ischemia-reperfusion model”, Biomaterials 74, 19-30 (2016). link
  • U. Chitgupi, Y. M. Zhang, C. Y. Lo, S. Shao, W. T. Song, J. M. Geng, S. Neelamegham, J. F. Lovell, “Sulfonated Polyethylenimine for Photosensitizer Conjugation and Targeting”, Bioconjugate Chem. 26, 1633-1639 (2015). link
  • G. Liu, S. Neelamegham, “Integration of systems glycobiology with bioinformatics toolboxes, glycoinformatics resources, and glycoproteomics data”, Wiley Interdiscip. Rev.-Syst. Biol 7, 163-181 (2015). link
  • S. Gogia, C. Y. Lo, S. Neelamegham, “Detection of Plasma Protease Activity Using Microsphere-Cytometry Assays with E-coli Derived Substrates: VWF Proteolysis by ADAMTS13”, PLoS One 10, e0126556 (2015). link
  • S. Shao, J. M. Geng, H. A. Yi, S. Gogia, S. Neelamegham, A. Jacobs, J. F. Lovell, “Functionalization of cobalt porphyrin-phospholipid bilayers with his-tagged ligands and antigens”, Nat. Chem. 7, 438-446 (2015). link
  • N. Mondal, A. Buffone, G. Stolfa, A. Antonopoulos, J. T. Y. Lau, S. M. Haslam, A. Dell, S. Neelamegham, “ST3Gal-4 is the primary sialyltransferase regulating the synthesis of E-, P-, and L-selectin ligands on human myeloid leukocytes”, Blood 125, 687-696 (2015). link
  • S. R. Madabhushi, C. J. Zhang, A. Kelkar, K. M. Dayananda, S. Neelamegham, “Platelet Gplb alpha Binding to von Willebrand Factor Under Fluid Shear: Contributions of the D'D3-Domain, A1-Domain Flanking Peptide and O-Linked Glycans”, J. Am. Heart Assoc. 3, e001420 (2014). link
  • G. Liu, S. Neelamegham, “A Computational Framework for the Automated Construction of Glycosylation Reaction Networks”, PLoS One 9, e100939 (2014). link
  • N. Mondal, A. Buffone, S. Neelamegham, “Distinct glycosyltransferases synthesize E-selectin ligands in human vs. mouse leukocytes”, Celll Adhes. Migr. 7, 288-292 (2013). link PDF
  • C. Y. Lo, A. Antonopoulos, A. Dell, S. M. Haslam, T. Lee, S. Neelamegham, “The use of surface immobilization of P-selectin glycoprotein ligand-1 on mesenchymal stem cells to facilitate selectin mediated cell tethering and rolling”, Biomaterials 34, 8213-8222 (2013). link PDF
  • C. Y. Lo, A. Antonopoulos, R. Gupta, J. Qu, A. Dell, S. M. Haslam, S. Neelamegham, “Competition between Core-2 GlcNAc-transferase and ST6GalNAc-transferase Regulates the Synthesis of the Leukocyte Selectin Ligand on Human P-selectin Glycoprotein Ligand-1”, J. Biol. Chem. 288, 13974-13987 (2013). link PDF
  • G. Liu, A. Puri, S. Neelamegham, “Glycosylation Network Analysis Toolbox: a MATLAB-based environment for systems glycobiology”, Bioinformatics 29, 404-406 (2013). link
  • A. Buffone, N. Mondal, R. Gupta, K. P. Mchugh, J. T. Y. Lau, S. Neelamegham, “Silencing alpha 1,3-Fucosyltransferases in Human Leukocytes Reveals a Role for FUT9 Enzyme during E-selectin-mediated Cell Adhesion”, J. Biol. Chem. 288, 1620-1633 (2013). link
  • S. R. Madabhushi, C. W. Shang, K. M. Dayananda, K. Rittenhouse-olson, M. Murphy, T. E. Ryan, R. R. Montgomery, S. Neelamegham, “von Willebrand factor (VWF) propeptide binding to VWF D ' D3 domain attenuates platelet activation and adhesion”, Blood 119, 4769-4778 (2012). link
  • E. V. Chandrasekaran, J. Xue, J. Xia, R. D. Locke, S. A. Patil, S. Neelamegham, K. L. Matta, “Characterization of Cancer Associated Mucin Type O-Glycans Using the Exchange Sialylation Properties of Mammalian Sialyltransferase ST3Gal-II”, J. Proteome Res. 11, 2609-2618 (2012). link
  • S. Chinthamani, O. Odusanwo, N. Mondal, J. Nelson, S. Neelamegham, O. J. Baker, “Lipoxin A(4) inhibits immune cell binding to salivary epithelium and vascular endothelium”, Am. J. Physiol.-Cell Physiol. 302, C968-C978 (2012). link
  • A. Puri, S. Neelamegham, “Understanding Glycomechanics Using Mathematical Modeling: A Review of Current Approaches to Simulate Cellular Glycosylation Reaction Networks”, Ann. Biomed. Eng. 40, 816-827 (2012). link
  • S. I. Simon, S. Neelamegham, K. Konstantopoulos, “Preface to Special Issue: "Glycomechanics: Sugar Coating Blood Cell-Endothelial Interactions in Shear Flow"”, Ann. Biomed. Eng. 40, 764-765 (2012). link
  • S. A. Patil, E. V. Chandrasekaran, K. L. Matta, A. Parikh, E. S. Tzanakakis, S. Neelamegham, “Scaling down the size and increasing the throughput of glycosyltransferase assays: Activity changes on stem cell differentiation”, Anal. Biochem. 425, 135-144 (2012). link/PDF
  • S. Neelamegham, G. Liu, “Systems glycobiology: biochemical reaction networks regulating glycan structure and function”, Glycobiology 21, 1541-1553 (2011). link
  • E. V. Chandrasekaran, J. Xue, J. Xia, R. D. Locke, S. A. Patil, S. Neelamegham, K. L. Matta, “Mammalian Sialyltransferase ST3Gal-II: Its Exchange Sialylation Catalytic Properties Allow Labeling of Sialyl Residues in Mucin-Type Sialylated Glycoproteins and Specific Gangliosides”, Biochemistry 50, 9475-9487 (2011). link PDF
  • K. M. Dayananda, S. Gogia, S. Neelamegham, “Escherichia coli-derived von Willebrand factor-A2 domain fluorescence/Forster resonance energy transfer proteins that quantify ADAMTS13 activity”, Anal. Biochem. 410, 206-213 (2011). link PDF
  • S. R. Barthel, A. Antonopoulos, F. Cedeno-laurent, L. Schaffer, G. Hernandez, S. A. Patil, S. J. North, A. Dell, K. L. Matta, S. Neelamegham, S. M. Haslam, C. J. Dimitroff, “Peracetylated 4-Fluoro-glucosamine Reduces the Content and Repertoire of N- and O-Glycans without Direct Incorporation”, J. Biol. Chem. 286, 21717-21731 (2011). link PDF
  • D. D. Marathe, A. Buffone, E. V. Chandrasekaran, J. Xue, R. D. Locke, M. Nasirikenari, J. T. Y. Lau, K. L. Matta, S. Neelamegham, “Fluorinated per-acetylated GalNAc metabolically alters glycan structures on leukocyte PSGL-1 and reduces cell binding to selectins”, Blood 115, 1303-1312 (2010). link PDF
  • K. M. Dayananda, I. Singh, N. Mondal, S. Neelamegham, “von Willebrand factor self-association on platelet GpIb alpha under hydrodynamic shear: effect on shear-induced platelet activation”, Blood 116, 3990-3998 (2010). link PDF
  • K. H. Lim, S. R. Madabhushi, J. Mann, S. Neelamegham, S. Park, “Disulfide Trapping of Protein Complexes on the Yeast Surface”, Biotechnol. Bioeng. 106, 27-41 (2010). link
  • I. Singh, E. Themistou, L. Porcar, S. Neelamegham, “Fluid Shear Induces Conformation Change in Human Blood Protein von Willebrand Factor in Solution”, Biophys. J. 96, 2313-2320 (2009). link PDF
  • D. Jayakumar, D. D. Marathe, S. Neelamegham, “Detection of Site-Specific Glycosylation in Proteins Using Flow Cytometry”, Cytom. Part A 75A, 866-873 (2009). link PDF
  • E. Themistou, I. Singh, C. W. Shang, S. V. Balu-iyer, P. Alexandridis, S. Neelamegham, “Application of Fluorescence Spectroscopy to Quantify Shear-Induced Protein Conformation Change”, Biophys. J. 97, 2567-2576 (2009). link PDF
  • S. L. Diamond, M. B. Lawrence, S. Neelamegham, “Harry L. Goldsmith, Ph.D.”, Ann. Biomed. Eng. 36, 523-526 (2008). link
  • Z. H. Xiao, G. P. Visentin, K. M. Dayananda, S. Neelamegham, “Immune complexes formed following the binding of anti-platelet factor 4 (CXCL4) antibodies to CXCL4 stimulate human neutrophil activation and cell adhesion”, Blood 112, 1091-1100 (2008). link PDF
  • D. D. Marathe, E. V. Chandrasekaran, J. T. Y. Lau, K. L. Matta, S. Neelamegham, “Systems-level studies of glycosyltransferase gene expression and enzyme activity that are associated with the selectin binding function of human leukocytes”, Faseb J. 22, 4154-4167 (2008). link PDF
  • G. Liu, D. D. Marathe, K. L. Matta, S. Neelamegham, “Systems-level modeling of cellular glycosylation reaction networks: O-linked glycan formation on natural selectin ligands”, Bioinformatics 24, 2740-2747 (2008). link PDF
  • E. V. Chandrasekaran, J. Xue, J. Xia, R. D. Locke, K. L. Matta, S. Neelamegham, “Reversible sialylation: Synthesis of cytidine 5 '-monophospho-N-acetylneuraminic acid from cytidine 5 '-monophosphate with alpha 2,3-sialyl O-glycan-, glycolipid-, and macromolecule-based donors yields diverse sialylated products”, Biochemistry 47, 320-330 (2008). link PDF
  • Y. Zhang, H. N. Hayenga, M. R. Sarantos, S. I. Simon, S. Neelamegham, “Differential regulation of neutrophil CD18 integrin function by di- and tri-valent cations: Manganese vs. gadolinium”, Ann. Biomed. Eng. 36, 647-660 (2008). link PDF
  • G. Liu, S. Neelamegham, “In silico Biochemical Reaction Network Analysis (IBRENA): a package for simulation and analysis of reaction networks”, Bioinformatics 24, 1109-1111 (2008). link PDF
  • E. V. Chandrasekaran, J. Xue, S. Neelamegham, K. L. Matta, “The pattern of glycosyl- and sulfotransferase activities in cancer cell lines: a predictor of individual cancer-associated distinct carbohydrate structures for the structural identification of signature glycans”, Carbohydr. Res. 341, 983-994 (2006). link PDF
  • Z. H. Xiao, H. L. Goldsmith, F. A. Mcintosh, H. Shankaran, S. Neelamegham, “Biomechanics of P-selectin PSGL-1 bonds: Shear threshold and integrin-independent cell adhesion”, Biophys. J. 90, 2221-2234 (2006). link PDF
  • I. Singh, H. Shankaran, M. E. Beauharnois, Z. H. Xiao, P. Alexandridis, S. Neelamegham, “Solution structure of human von Willebrand factor studied using small angle neutron scattering”, J. Biol. Chem. 281, 38266-38275 (2006). link PDF
  • E. V. Chandrasekaran, J. Xue, J. Xia, R. Chawda, C. Piskorz, R. D. Locke, S. Neelamegham, K. L. Matta, “Analysis of the specificity of sialyltransferases toward mucin core 2, Globo, and related structures. Identification of the sialylation sequence and the effects of sulfate, fucose, methyl, and fluoro substituents of the carbohydrate chain in the biosynthesis of selectin and siglec ligands, and novel sialylation by cloned alpha 2,3(O)sialyltransferase”, Biochemistry 44, 15619-15635 (2005). link/PDF
  • G. Liu, M. T. Swihart, S. Neelamegham, “Sensitivity, principal component and flux analysis applied to signal transduction: the case of epidermal growth factor mediated signaling”, Bioinformatics 21, 1194-1202 (2005). link PDF
  • M. E. Beauharnois, K. C. Lindquist, D. Marathe, P. Vanderslice, J. Xia, K. L. Matta, S. Neelamegham, “Affinity and kinetics of sialyl Lewis-X and core-2 based oligosaccharides binding to L- and P-selectin”, Biochemistry 44, 9507-9519 (2005). link PDF
  • S. Neelamegham, “Transport features, reaction kinetics and receptor biomechanics controlling selectin and integrin mediated cell adhesion”, Cell Commun. Adhes. 11, 35-50 (2004). link PDF
  • H. Shankaran, S. Neelamegham, “Hydrodynamic forces applied on intercellular bonds, soluble molecules, and cell-surface receptors”, Biophys. J. 86, 576-588 (2004). link PDF
  • E. V. Chandrasekaran, S. S. Lakhaman, R. Chawda, C. F. Piskorz, S. Neelamegham, K. L. Matta, “Identification of physiologically relevant substrates for cloned Gal : 3-O-sulfotransferases (Gal3STs) - Distinct high affinity of Gal3ST-2 and LS180 sulfotransferase for the Globo H backbone, Gal3ST-3 for N-glycan multiterminal Gal beta 1,4GlcNAc beta units and 6-sulfoGal beta 1,4GlcNAc beta, and Gal3ST-4 for the mucin core-2 trisaccharide”, J. Biol. Chem. 279, 10032-10041 (2004). link PDF
  • D. B. Mcdonough, F. A. Mcintosh, C. Spanos, S. Neelamegham, H. L. Goldsmith, S. I. Simon, “Cooperativity between selectins and beta(2)-integrins define neutrophil capture and stable adhesion in shear flow”, Ann. Biomed. Eng. 32, 1179-1192 (2004). link PDF
  • H. Shankaran, P. Alexandridis, S. Neelamegham, “Aspects of hydrodynamic shear regulating shear-induced platelet activation and self-association of von Willebrand factor in suspension”, Blood 101, 2637-2645 (2003). link PDF
  • Y. Zhang, S. Neelamegham, “Response to the letter by King and Hammer”, Biophys. J. 84, 4183-4183 (2003).
  • Y. Zhang, S. Neelamegham, “PPLATE: a computer program for analysis of parallel-plate flow chamber experimental data”, J. Immunol. Methods 278, 319-321 (2003). link PDF
  • Y. Zhang, S. Neelamegham, “An analysis tool to quantify the efficiency of cell tethering and firm-adhesion in the parallel-plate flow chamber”, J. Immunol. Methods 278, 305-317 (2003). link PDF
  • Y. Zhang, S. Neelamegham, “Estimating the efficiency of cell capture and arrest in flow chambers: Study of neutrophil binding via E-selectin and ICAM-1”, Biophys. J. 83, 1934-1952 (2002). PDF
  • H. Shankaran, S. Neelamegham, “Effect of secondary flow on biological experiments in the cone-plate viscometer: Methods for estimating collision frequency, wall shear stress and inter-particle interactions in non-linear flow”, Biorheology 38, 275-304 (2001). PDF
  • F. Ahmed, P. Alexandridis, S. Neelamegham, “Synthesis and application of fluorescein-labeled Pluronic block copolymers to the study of polymer-surface interactions”, Langmuir 17, 537-546 (2001). link PDF
  • H. Shankaran, S. Neelamegham, “Nonlinear flow affects hydrodynamic forces and neutrophil adhesion rates in cone-plate viscometers”, Biophys. J. 80, 2631-2648 (2001).
  • F. Ahmed, P. Alexandridis, H. Shankaran, S. Neelamegham, “The ability of poloxamers to inhibit platelet aggregation depends on their physicochemical properties”, Thromb. Haemost. 86, 1532-1539 (2001).
  • E. R. Hentzen, S. Neelamegham, G. S. Kansas, J. A. Benanti, L. V. Mcintire, C. W. Smith, S. I. Simon, “Sequential binding of CD11a/CD18 and CD11b/CD18 defines neutrophil capture and stable adhesion to Intercellular adhesion molecule-1”, Blood 95, 911-920 (2000).
  • S. Neelamegham, A. D. Taylor, H. Shankaran, C. W. Smith, S. I. Simon, “Shear and time-dependent changes in Mac-1, LFA-1, and ICAM-3 binding regulate neutrophil homotypic adhesion”, J. Immunol. 164, 3798-3805 (2000). PDF
  • K. Konstantopoulos, S. Neelamegham, A. R. Burns, E. Hentzen, G. S. Kansas, K. R. Snapp, E. L. Berg, J. D. Hellums, C. W. Smith, L. V. Mcintire, S. I. Simon, “Venous levels of shear support neutrophil-platelet adhesion and neutrophil aggregation in blood via P-selectin and beta(2)-integrin”, Circulation 98, 873-882 (1998).
  • E. Lynam, L. A. Sklar, A. D. Taylor, S. Neelamegham, B. S. Edwards, C. W. Smith, S. I. Simon, “beta(2)-integrins mediate stable adhesion in collisional interactions between neutrophils and ICAM-1-expressing cells”, J. Leukoc. Biol. 64, 622-630 (1998).
  • S. I. Simon, S. Neelamegham, A. Taylor, C. W. Smith, “The multistep process of homotypic neutrophil aggregation: A review of the molecules and effects of hydrodynamics”, Cell Adhes. Commun. 6, 263-276 (1998). link
  • S. Neelamegham, A. D. Taylor, A. R. Burns, C. W. Smith, S. I. Simon, “Hydrodynamic shear shows distinct roles for LFA-1 and Mac-1 in neutrophil adhesion to intercellular adhesion molecule-1”, Blood 92, 1626-1638 (1998).
  • S. Neelamegham, K. Zygourakis, “A quantitative assay for intercellular aggregation”, Ann. Biomed. Eng. 25, 180-189 (1997). link PDF
  • S. Neelamegham, L. L. Munn, K. Zygourakis, “Model for the kinetics of homotypic cellular aggregation under static conditions”, Biophys. J. 72, 51-64 (1997). PDF
  • S. Neelamegham, A. D. Taylor, J. D. Hellums, M. Dembo, C. W. Smith, S. I. Simon, “Modeling the reversible kinetics of neutrophil aggregation under hydrodynamic shear”, Biophys. J. 72, 1527-1540 (1997).
  • B. W. Mcintyre, D. G. Woodside, D. A. Caruso, D. K. Wooten, S. I. Simon, S. Neelamegham, J. K. Revelle, P. Vanderslice, “Regulation of human T lymphocyte coactivation with an alpha(4) integrin antagonist peptide”, J. Immunol. 158, 4180-4186 (1997).
  • Y. T. M. Tsang, S. Neelamegham, Y. Hu, E. L. Berg, A. R. Burns, C. W. Smith, S. I. Simon, “Synergy between L-selectin signaling and chemotactic activation during neutrophil adhesion and transmigration”, J. Immunol. 159, 4566-4577 (1997).
  • S. Neelamegham, S. I. Simon, B. W. Mcintyre, K. Zygourakis, “Induction of homotypic lymphocyte aggregation: Evidence for a novel activation state of the beta(1) integrin”, J. Leukoc. Biol. 59, 872-882 (1996). PDF
  • A. D. Taylor, S. Neelamegham, J. D. Hellums, C. W. Smith, S. I. Simon, “Molecular dynamics of the transition from L-selectin- to beta(2)-integrin-dependent neutrophil adhesion under defined hydrodynamic shear”, Biophys. J. 71, 3488-3500 (1996).
  • A. R. Ray, S. Neelamegham, A. Bhowmick, “Physicochemical studies of polyetherurethane”, Polym. Int. 24, 149-157 (1991). link

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