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NANOG Reverses the Myogenic Differentiation Potential of Senescent Stem Cells by Restoring ACTIN Filamentous Organization and SRF-Dependent Gene Expression - Mistriotis - 2016 - STEM CELLS - Wiley Online Library
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NANOG Reverses the Myogenic Differentiation Potential of Senescent Stem Cells by Restoring ACTIN Filamentous Organization and SRF-Dependent Gene Expression

Authors

  • Panagiotis Mistriotis,

    1. Bioengineering Laboratory, Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Amherst, New York, USA
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  • Vivek K. Bajpai,

    1. Bioengineering Laboratory, Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Amherst, New York, USA
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  • Xiaoyan Wang,

    1. Bioengineering Laboratory, Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Amherst, New York, USA
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  • Na Rong,

    1. Bioengineering Laboratory, Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Amherst, New York, USA
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  • Aref Shahini,

    1. Bioengineering Laboratory, Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Amherst, New York, USA
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  • Mohammadnabi Asmani,

    1. Department of Biomedical Engineering, University at Buffalo, The State University of New York, Amherst, New York, USA
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  • Mao-Shih Liang,

    1. Bioengineering Laboratory, Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Amherst, New York, USA
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  • Jianmin Wang,

    1. Department of Biostatistics and Bioinformatics, Roswell Park Cancer Institute, Buffalo, New York, USA
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  • Pedro Lei,

    1. Bioengineering Laboratory, Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Amherst, New York, USA
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  • Song Liu,

    1. Department of Biostatistics and Bioinformatics, Roswell Park Cancer Institute, Buffalo, New York, USA
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  • Ruogang Zhao,

    1. Department of Biomedical Engineering, University at Buffalo, The State University of New York, Amherst, New York, USA
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  • Stelios T. Andreadis

    Corresponding author
    1. Bioengineering Laboratory, Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Amherst, New York, USA
    2. Department of Biomedical Engineering, University at Buffalo, The State University of New York, Amherst, New York, USA
    • Correspondence: Stelios Andreadis, Ph.D., Professor and Chair, Bioengineering Laboratory, 908 Furnas Hall, Department of Chemical and Biological Engineering, Department of Biomedical Engineering, and Center of Excellence in Bioinformatics and Life Sciences, University at Buffalo, The State University of New York, Amherst, New York 14260-4200, USA. Telephone: (716) 645-1202; Fax: (716) 645-3822; e-mail: sandread@buffalo.edu

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Abstract

Cellular senescence as a result of organismal aging or progeroid diseases leads to stem cell pool exhaustion hindering tissue regeneration and contributing to the progression of age related disorders. Here we discovered that ectopic expression of the pluripotent factor NANOG in senescent or progeroid myogenic progenitors reversed cellular aging and restored completely the ability to generate contractile force. To elicit its effects, NANOG enabled reactivation of the ROCK and Transforming Growth Factor (TGF)-β pathways—both of which were impaired in senescent cells—leading to ACTIN polymerization, MRTF-A translocation into the nucleus and serum response factor (SRF)-dependent myogenic gene expression. Collectively our data reveal that cellular senescence can be reversed and provide a novel strategy to regain the lost function of aged stem cells without reprogramming to the pluripotent state. Stem Cells 2017;35:207–221